Detailed information of TA system TA073645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 685868..687111
• Replicon: chromosome
• Accession: NZ_CP019978
• Organism: Streptococcus agalactiae strain Sag37

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: B1H24_RS03850
• Protein ID: WP_001235242.1
• Coordinates: 686344..687111 (+)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: B1H24_RS03845
• Protein ID: WP_000578719.1
• Coordinates: 685868..686344 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H24_RS03820	681961..683652	+	1692	WP_050547274.1	recombinase family protein	-
B1H24_RS03825	683716..684816	+	1101	WP_000196859.1	toprim domain-containing protein	-
B1H24_RS03830	684844..685071	+	228	WP_000110922.1	hypothetical protein	-
B1H24_RS03835	685068..685457	+	390	WP_000048066.1	hypothetical protein	-
B1H24_RS03840	685508..685798	+	291	WP_000667951.1	hypothetical protein	-
B1H24_RS03845	685868..686344	+	477	WP_000578719.1	helix-turn-helix domain-containing protein	Antitoxin
B1H24_RS03850	686344..687111	+	768	WP_001235242.1	zeta toxin family protein	Toxin
B1H24_RS03855	687148..689655	+	2508	WP_001213955.1	AAA family ATPase	-
B1H24_RS03865	689993..690355	+	363	WP_000140674.1	hypothetical protein	-
B1H24_RS03870	690358..690723	+	366	WP_000431640.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / aph(3')-III / erm(B)	gbs0628 / gbs0628 / gbs0630	607227..713468	106241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T73645 WP_001235242.1 NZ_CP019978:686344-687111 [Streptococcus agalactiae]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T73645 NZ_CP019978:686344-687111 [Streptococcus agalactiae]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT73645 WP_000578719.1 NZ_CP019978:685868-686344 [Streptococcus agalactiae]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT73645 NZ_CP019978:685868-686344 [Streptococcus agalactiae]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCATCTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCAAAGCTTTATAGATACCAGGATAGTCAAGATATTGTTTTTGATGATGA
ATCTAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATCAATACGAAAATTTACCTTGTCGCTACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGATGGTATAGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: G7SEB4