Detailed information of TA system TA073644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 676729..677161
• Replicon: chromosome
• Accession: NZ_CP019978
• Organism: Streptococcus agalactiae strain Sag37

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: B1H24_RS03780
• Protein ID: WP_001284310.1
• Coordinates: 677003..677161 (+)
• Length: 53 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: B1H24_RS03775
• Protein ID: WP_000301765.1
• Coordinates: 676729..677001 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H24_RS03735	672355..672606	+	252	Protein_636	GNAT family N-acetyltransferase	-
B1H24_RS03740	672699..673493	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
B1H24_RS03745	673870..673953	+	84	Protein_638	peptide-binding protein	-
B1H24_RS03750	674002..674085	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
B1H24_RS03755	674210..674947	+	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
B1H24_RS03760	675093..675377	+	285	WP_073459356.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
B1H24_RS03765	675508..676404	+	897	WP_001809760.1	ParA family protein	-
B1H24_RS03770	676502..676711	+	210	WP_000527318.1	peptide-binding protein	-
B1H24_RS03775	676729..677001	+	273	WP_000301765.1	antitoxin	Antitoxin
B1H24_RS03780	677003..677161	+	159	WP_001284310.1	zeta toxin family protein	Toxin
B1H24_RS03785	677213..677758	+	546	WP_001071501.1	AAA family ATPase	-
B1H24_RS03790	677934..678800	+	867	WP_001255868.1	aminoglycoside 6-adenylyltransferase AadE	-
B1H24_RS03805	679371..680039	+	669	Protein_648	zeta toxin family protein	-
B1H24_RS03810	680190..680672	+	483	Protein_649	hypothetical protein	-
B1H24_RS03815	680673..681838	+	1166	Protein_650	type IV secretory system conjugative DNA transfer family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / aph(3')-III / erm(B)	gbs0628 / gbs0628 / gbs0630	607227..713468	106241
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	669849..678800	8951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (26-50)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5790.55 Da
• Isoelectric Point: 10.3088

>T73644 WP_001284310.1 NZ_CP019978:677003-677161 [Streptococcus agalactiae]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRRR

Nucleotide

• Length: 159 bp

>T73644 NZ_CP019978:677003-677161 [Streptococcus agalactiae]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGAAGAAGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT73644 WP_000301765.1 NZ_CP019978:676729-677001 [Streptococcus agalactiae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT73644 NZ_CP019978:676729-677001 [Streptococcus agalactiae]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• PDB: 3Q8X
• PDB: 1GVN
• AlphaFold DB: A0A829F0A3