Detailed information of TA system TA073643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 667993..669101
• Replicon: chromosome
• Accession: NZ_CP019978
• Organism: Streptococcus agalactiae strain Sag37

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A193AUR0
• Locus tag: B1H24_RS03710
• Protein ID: WP_002378351.1
• Coordinates: 668232..669101 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: B1H24_RS03705
• Protein ID: WP_000205227.1
• Coordinates: 667993..668217 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H24_RS03675	663205..663378	+	174	WP_002779755.1	cysteine-rich KTR domain-containing protein	-
B1H24_RS11780	663581..663751	+	171	WP_000713595.1	hypothetical protein	-
B1H24_RS03680	663765..664406	+	642	WP_154021687.1	recombinase family protein	-
B1H24_RS03685	664384..665061	+	678	Protein_626	DNA topoisomerase	-
B1H24_RS03690	665573..665869	+	297	WP_002360685.1	hypothetical protein	-
B1H24_RS03695	665870..666286	+	417	WP_000323438.1	recombinase	-
B1H24_RS03700	666288..667850	+	1563	WP_000136908.1	recombinase family protein	-
B1H24_RS03705	667993..668217	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
B1H24_RS03710	668232..669101	+	870	WP_002378351.1	nucleotidyltransferase domain-containing protein	Toxin
B1H24_RS03715	669082..669816	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
B1H24_RS03720	669849..670757	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
B1H24_RS03725	670754..670960	+	207	WP_044116541.1	hypothetical protein	-
B1H24_RS03730	671034..672196	+	1163	WP_088186165.1	IS3-like element ISSag12 family transposase	-
B1H24_RS03735	672355..672606	+	252	Protein_636	GNAT family N-acetyltransferase	-
B1H24_RS03740	672699..673493	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
B1H24_RS03745	673870..673953	+	84	Protein_638	peptide-binding protein	-
B1H24_RS03750	674002..674085	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / aph(3')-III / erm(B)	gbs0628 / gbs0628 / gbs0630	607227..713468	106241
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	669849..678800	8951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32912.50 Da
• Isoelectric Point: 4.7903

>T73643 WP_002378351.1 NZ_CP019978:668232-669101 [Streptococcus agalactiae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T73643 NZ_CP019978:668232-669101 [Streptococcus agalactiae]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAGCTTTATAATGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT73643 WP_000205227.1 NZ_CP019978:667993-668217 [Streptococcus agalactiae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT73643 NZ_CP019978:667993-668217 [Streptococcus agalactiae]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: A0A193AUR0