Detailed information of TA system TA073640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2460..3067
• Replicon: plasmid p114-4
• Accession: NZ_CP019974
• Organism: Enterococcus faecium isolate 2014-VREF-114

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: B4U48_RS16140
• Protein ID: WP_002321032.1
• Coordinates: 2460..2810 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: B4U48_RS16145
• Protein ID: WP_002287514.1
• Coordinates: 2804..3067 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4U48_RS16120	1..811	+	811	Protein_0	replication initiation factor domain-containing protein	-
B4U48_RS16125	852..1046	+	195	WP_016922260.1	hypothetical protein	-
B4U48_RS17060	1039..1170	+	132	WP_153302735.1	helix-turn-helix domain-containing protein	-
B4U48_RS16130	1208..1888	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
B4U48_RS16135	1930..2436	+	507	WP_079158060.1	recombinase family protein	-
B4U48_RS16140	2460..2810	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B4U48_RS16145	2804..3067	-	264	WP_002287514.1	hypothetical protein	Antitoxin
B4U48_RS16150	3408..3587	-	180	WP_002305761.1	hypothetical protein	-
B4U48_RS16160	3968..4675	-	708	WP_002353391.1	hypothetical protein	-
B4U48_RS16165	4668..5105	-	438	WP_002296237.1	hypothetical protein	-
B4U48_RS16170	5120..5371	-	252	WP_002296238.1	hypothetical protein	-
B4U48_RS16175	5371..5577	-	207	WP_002296239.1	hypothetical protein	-
B4U48_RS16185	6018..6278	-	261	WP_002296240.1	hypothetical protein	-
B4U48_RS16190	6644..7129	-	486	WP_002296241.1	single-stranded DNA-binding protein	-
B4U48_RS16195	7132..8031	-	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55063	55063
	-	flank	IS/Tn	-	-	1202..1888	686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T73640 WP_002321032.1 NZ_CP019974:c2810-2460 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T73640 NZ_CP019974:c2810-2460 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT73640 WP_002287514.1 NZ_CP019974:c3067-2804 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT73640 NZ_CP019974:c3067-2804 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0