Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 36822..37429 Replicon plasmid p114-3
Accession NZ_CP019973
Organism Enterococcus faecium isolate 2014-VREF-114

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag B4U48_RS15990 Protein ID WP_002321032.1
Coordinates 36822..37172 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag B4U48_RS15995 Protein ID WP_002287514.1
Coordinates 37166..37429 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4U48_RS15955 32057..32581 - 525 WP_079158058.1 helix-turn-helix transcriptional regulator -
B4U48_RS15960 32631..33266 - 636 WP_010730987.1 LCP family protein -
B4U48_RS15965 33328..34008 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
B4U48_RS15970 34204..35172 + 969 WP_045135958.1 replication initiation factor domain-containing protein -
B4U48_RS15975 35175..35408 + 234 WP_002298421.1 hypothetical protein -
B4U48_RS17055 35401..35532 + 132 WP_153302735.1 helix-turn-helix domain-containing protein -
B4U48_RS15980 35570..36250 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
B4U48_RS15985 36292..36798 + 507 WP_079158060.1 recombinase family protein -
B4U48_RS15990 36822..37172 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B4U48_RS15995 37166..37429 - 264 WP_002287514.1 hypothetical protein Antitoxin
B4U48_RS16000 37770..37949 - 180 WP_002305761.1 hypothetical protein -
B4U48_RS16010 38330..39037 - 708 WP_002353391.1 hypothetical protein -
B4U48_RS16015 39030..39467 - 438 WP_002296237.1 hypothetical protein -
B4U48_RS16020 39482..39733 - 252 WP_002296238.1 hypothetical protein -
B4U48_RS16025 39733..39939 - 207 WP_002296239.1 hypothetical protein -
B4U48_RS16035 40380..40640 - 261 WP_002296240.1 hypothetical protein -
B4U48_RS16040 41006..41491 - 486 WP_002296241.1 single-stranded DNA-binding protein -
B4U48_RS16045 41494..42393 - 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX - 1..56285 56285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T73639 WP_002321032.1 NZ_CP019973:c37172-36822 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T73639 NZ_CP019973:c37172-36822 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT73639 WP_002287514.1 NZ_CP019973:c37429-37166 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT73639 NZ_CP019973:c37429-37166 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References