Detailed information of TA system TA073628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4491539..4492191
• Replicon: chromosome
• Accession: NZ_CP019966
• Organism: Rhodopseudomonas palustris strain PS3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RPPS3_RS20810
• Protein ID: WP_107345758.1
• Coordinates: 4491787..4492191 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6N2A9
• Locus tag: RPPS3_RS20805
• Protein ID: WP_011159676.1
• Coordinates: 4491539..4491790 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPPS3_RS20795	4486876..4487259	-	384	WP_107345756.1	phasin	-
RPPS3_RS20800	4487584..4491162	+	3579	WP_107345757.1	PAS domain-containing protein	-
RPPS3_RS20805	4491539..4491790	+	252	WP_011159676.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RPPS3_RS20810	4491787..4492191	+	405	WP_107345758.1	type II toxin-antitoxin system VapC family toxin	Toxin
RPPS3_RS20815	4492275..4492985	+	711	WP_107345759.1	Crp/Fnr family transcriptional regulator	-
RPPS3_RS20820	4492958..4493182	-	225	WP_107345760.1	DUF1858 domain-containing protein	-
RPPS3_RS20825	4493420..4494532	+	1113	WP_107345761.1	nitrite reductase, copper-containing	-
RPPS3_RS20830	4494685..4495566	+	882	WP_107345762.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Antitoxin VapB-like (1-82)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14419.58 Da
• Isoelectric Point: 5.7209

>T73628 WP_107345758.1 NZ_CP019966:4491787-4492191 [Rhodopseudomonas palustris]
VIVIDTSAIIAIFREEPEAAQFARLIASDDQPVLSSGNLLEAAIALRGLKDIQPAKAERWLDDFIAAARIRIEPVTTEQA
GYARSAHLRFGKGTGHKAALNYGDCFAYALAKALNAPLLCKGNDFPLTDIPLVT

Nucleotide

• Length: 405 bp

>T73628 NZ_CP019966:4491787-4492191 [Rhodopseudomonas palustris]
GTGATCGTCATCGACACGTCGGCGATCATTGCGATCTTTCGCGAGGAACCAGAGGCAGCCCAGTTTGCGCGGCTCATCGC
CTCCGACGACCAACCGGTTCTCTCCTCGGGCAATTTGCTGGAGGCCGCGATCGCTCTTCGTGGACTAAAGGACATTCAGC
CAGCCAAAGCCGAACGATGGCTTGATGACTTCATCGCGGCGGCCCGCATCCGGATCGAGCCCGTCACCACCGAGCAGGCC
GGATACGCGCGTTCGGCGCATCTGCGGTTCGGGAAAGGGACCGGCCACAAAGCAGCTCTCAACTACGGAGATTGCTTCGC
CTATGCGCTGGCCAAAGCTTTGAATGCCCCCCTGCTGTGCAAGGGCAACGACTTTCCGCTGACCGATATTCCGCTTGTCA
CGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9293.42 Da
• Isoelectric Point: 6.7249

>AT73628 WP_011159676.1 NZ_CP019966:4491539-4491790 [Rhodopseudomonas palustris]
MHLNIKNDEAHQLATELARMTGESLTAAVTNALREQLAREHRRRQTDQIAQRLLKIGGRYAALPDTGRTPDEILGYDENG
LPT

Nucleotide

• Length: 252 bp

>AT73628 NZ_CP019966:4491539-4491790 [Rhodopseudomonas palustris]
ATGCATCTCAACATCAAGAACGACGAAGCTCATCAGTTAGCGACAGAGCTGGCCCGCATGACCGGGGAGAGCCTCACTGC
GGCGGTGACCAATGCGCTGCGCGAACAGCTCGCCCGCGAGCATCGTCGCCGCCAAACCGACCAGATCGCGCAACGACTGC
TGAAAATCGGAGGCCGGTACGCCGCATTGCCTGACACCGGGAGAACTCCAGACGAGATTCTCGGCTACGATGAGAACGGA
TTGCCCACGTGA

Structures

Antitoxin

• AlphaFold DB: Q6N2A9