Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4834880..4835377 Replicon chromosome
Accession NZ_CP019953
Organism Escherichia coli M8

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag B2H83_RS23985 Protein ID WP_001173343.1
Coordinates 4835204..4835377 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag B2H83_RS23980 Protein ID WP_000398619.1
Coordinates 4834880..4835176 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2H83_RS23960 4831611..4832330 - 720 WP_000611288.1 two-component system response regulator DcuR -
B2H83_RS23965 4832327..4833958 - 1632 WP_001216461.1 sensor histidine kinase -
B2H83_RS23970 4834139..4834369 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
B2H83_RS23975 4834381..4834653 + 273 WP_000405647.1 N-acetyltransferase -
B2H83_RS23980 4834880..4835176 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
B2H83_RS23985 4835204..4835377 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
B2H83_RS23990 4835496..4837013 - 1518 WP_001295074.1 lysine--tRNA ligase -
B2H83_RS23995 4837250..4838707 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T73594 WP_001173343.1 NZ_CP019953:4835204-4835377 [Escherichia coli M8]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T73594 NZ_CP019953:4835204-4835377 [Escherichia coli M8]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT73594 WP_000398619.1 NZ_CP019953:4834880-4835176 [Escherichia coli M8]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT73594 NZ_CP019953:4834880-4835176 [Escherichia coli M8]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References