Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4024288..4025088 Replicon chromosome
Accession NZ_CP019953
Organism Escherichia coli M8

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag B2H83_RS20075 Protein ID WP_000342449.1
Coordinates 4024288..4024815 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag B2H83_RS20080 Protein ID WP_001277108.1
Coordinates 4024822..4025088 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2H83_RS20050 4019363..4020130 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
B2H83_RS20055 4020127..4021404 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
B2H83_RS20060 4021401..4022327 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
B2H83_RS20065 4022375..4023484 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
B2H83_RS20070 4023908..4024291 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
B2H83_RS20075 4024288..4024815 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
B2H83_RS20080 4024822..4025088 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
B2H83_RS20085 4025238..4026341 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
B2H83_RS20090 4026612..4027466 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
B2H83_RS20095 4027711..4028769 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
B2H83_RS20100 4028762..4029430 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T73592 WP_000342449.1 NZ_CP019953:c4024815-4024288 [Escherichia coli M8]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T73592 NZ_CP019953:c4024815-4024288 [Escherichia coli M8]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT73592 WP_001277108.1 NZ_CP019953:c4025088-4024822 [Escherichia coli M8]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT73592 NZ_CP019953:c4025088-4024822 [Escherichia coli M8]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References