Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3380238..3380892 Replicon chromosome
Accession NZ_CP019953
Organism Escherichia coli M8

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag B2H83_RS16795 Protein ID WP_000244772.1
Coordinates 3380238..3380645 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B2H83_RS16800 Protein ID WP_000354046.1
Coordinates 3380626..3380892 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2H83_RS16775 3376195..3377928 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
B2H83_RS16780 3377934..3378644 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B2H83_RS16785 3378669..3379565 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
B2H83_RS16790 3379677..3380198 + 522 WP_001055874.1 flavodoxin FldB -
B2H83_RS16795 3380238..3380645 - 408 WP_000244772.1 protein YgfX Toxin
B2H83_RS16800 3380626..3380892 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B2H83_RS16805 3381135..3382115 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
B2H83_RS16810 3382192..3382851 - 660 WP_000250274.1 hemolysin III family protein -
B2H83_RS16815 3383015..3383326 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
B2H83_RS16820 3383371..3384804 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
B2H83_RS16825 3384861..3385604 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T73589 WP_000244772.1 NZ_CP019953:c3380645-3380238 [Escherichia coli M8]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T73589 NZ_CP019953:c3380645-3380238 [Escherichia coli M8]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT73589 WP_000354046.1 NZ_CP019953:c3380892-3380626 [Escherichia coli M8]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT73589 NZ_CP019953:c3380892-3380626 [Escherichia coli M8]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References