Detailed information of TA system TA073587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2876490..2877115
• Replicon: chromosome
• Accession: NZ_CP019953
• Organism: Escherichia coli M8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B2H83_RS14255
• Protein ID: WP_000911330.1
• Coordinates: 2876490..2876888 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: B2H83_RS14260
• Protein ID: WP_000450524.1
• Coordinates: 2876888..2877115 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2H83_RS14235	2872368..2872568	+	201	WP_000383836.1	YpfN family protein	-
B2H83_RS14240	2872678..2873376	-	699	WP_000679823.1	esterase	-
B2H83_RS14245	2873450..2875465	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
B2H83_RS14250	2875480..2876343	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
B2H83_RS14255	2876490..2876888	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
B2H83_RS14260	2876888..2877115	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
B2H83_RS14265	2877269..2877982	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
B2H83_RS14270	2878195..2879229	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
B2H83_RS14275	2879246..2880124	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
B2H83_RS14280	2880270..2880842	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
B2H83_RS14285	2880842..2881312	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T73587 WP_000911330.1 NZ_CP019953:c2876888-2876490 [Escherichia coli M8]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T73587 NZ_CP019953:c2876888-2876490 [Escherichia coli M8]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT73587 WP_000450524.1 NZ_CP019953:c2877115-2876888 [Escherichia coli M8]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT73587 NZ_CP019953:c2877115-2876888 [Escherichia coli M8]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33