Detailed information of TA system TA073568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 583915..584752
• Replicon: chromosome
• Accession: NZ_CP019953
• Organism: Escherichia coli M8

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: B2H83_RS02745
• Protein ID: WP_000227784.1
• Coordinates: 583915..584457 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: B2H83_RS02750
• Protein ID: WP_001297137.1
• Coordinates: 584441..584752 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2H83_RS02725	579454..580365	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
B2H83_RS02730	580533..581024	+	492	WP_129541729.1	nucleotide binding protein YajQ	-
B2H83_RS02735	581152..582516	-	1365	WP_001000978.1	MFS transporter	-
B2H83_RS02740	582924..583859	+	936	WP_001297127.1	sel1 repeat family protein	-
B2H83_RS02745	583915..584457	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
B2H83_RS02750	584441..584752	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
B2H83_RS02755	584937..585827	-	891	WP_000971336.1	heme o synthase	-
B2H83_RS02760	585839..586168	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
B2H83_RS02765	586168..586782	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
B2H83_RS02770	586772..588763	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
B2H83_RS02775	588785..589732	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T73568 WP_000227784.1 NZ_CP019953:c584457-583915 [Escherichia coli M8]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T73568 NZ_CP019953:c584457-583915 [Escherichia coli M8]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT73568 WP_001297137.1 NZ_CP019953:c584752-584441 [Escherichia coli M8]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT73568 NZ_CP019953:c584752-584441 [Escherichia coli M8]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3