Detailed information of TA system TA073567

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 415107..415941
• Replicon: chromosome
• Accession: NZ_CP019953
• Organism: Escherichia coli M8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: B2H83_RS01975
• Protein ID: WP_079399123.1
• Coordinates: 415564..415941 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B2H83_RS01970
• Protein ID: WP_129541717.1
• Coordinates: 415107..415475 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2H83_RS01935	412026..412481	+	456	WP_000581502.1	hypothetical protein	-
B2H83_RS01940	412560..412793	+	234	WP_001119729.1	DUF905 family protein	-
B2H83_RS01950	412893..413675	+	783	WP_129541715.1	DUF945 domain-containing protein	-
B2H83_RS01955	413766..414251	+	486	WP_000849574.1	antirestriction protein	-
B2H83_RS01960	414267..414743	+	477	WP_001186726.1	RadC family protein	-
B2H83_RS01965	414806..415027	+	222	WP_129541716.1	DUF987 domain-containing protein	-
B2H83_RS01970	415107..415475	+	369	WP_129541717.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B2H83_RS01975	415564..415941	+	378	WP_079399123.1	type IV toxin-antitoxin system toxin CbtA	Toxin
B2H83_RS01980	415938..416426	+	489	WP_032215286.1	hypothetical protein	-
B2H83_RS01985	416446..416643	+	198	WP_000772024.1	DUF957 domain-containing protein	-
B2H83_RS01990	416740..417588	+	849	WP_129541718.1	DUF4942 domain-containing protein	-
B2H83_RS01995	417681..419000	-	1320	WP_000144688.1	site-specific integrase	-
B2H83_RS02000	419338..419748	-	411	WP_001111349.1	transcriptional regulator	-
B2H83_RS02005	419727..420683	-	957	WP_000121356.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14158.21 Da
• Isoelectric Point: 7.8523

>T73567 WP_079399123.1 NZ_CP019953:415564-415941 [Escherichia coli M8]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T73567 NZ_CP019953:415564-415941 [Escherichia coli M8]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13607.55 Da
• Isoelectric Point: 6.4652

>AT73567 WP_129541717.1 NZ_CP019953:415107-415475 [Escherichia coli M8]
VSDTLPGTILPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFALLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT73567 NZ_CP019953:415107-415475 [Escherichia coli M8]
GTGTCAGACACACTCCCCGGGACAATACTTCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTGCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA