Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 247104..247902 Replicon chromosome
Accession NZ_CP019953
Organism Escherichia coli M8

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag B2H83_RS01130 Protein ID WP_000854730.1
Coordinates 247104..247481 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag B2H83_RS01135 Protein ID WP_001285481.1
Coordinates 247528..247902 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2H83_RS01100 242902..243096 - 195 WP_001313570.1 DUF957 domain-containing protein -
B2H83_RS01105 243138..243429 + 292 Protein_210 transposase -
B2H83_RS01110 243426..243776 + 351 WP_000624707.1 IS66 family insertion sequence element accessory protein TnpB -
B2H83_RS01115 243807..245376 + 1570 Protein_212 IS66 family transposase -
B2H83_RS01120 245548..246536 + 989 Protein_213 IS630 family transposase -
B2H83_RS01125 246615..247107 - 493 Protein_214 hypothetical protein -
B2H83_RS01130 247104..247481 - 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
B2H83_RS01135 247528..247902 - 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
B2H83_RS01140 247952..248596 - 645 WP_000086759.1 hypothetical protein -
B2H83_RS01145 248615..248836 - 222 WP_000692315.1 DUF987 domain-containing protein -
B2H83_RS01150 248899..249375 - 477 WP_001313574.1 RadC family protein -
B2H83_RS01155 249391..249864 - 474 WP_001313575.1 antirestriction protein -
B2H83_RS01160 249958..250203 - 246 WP_001164966.1 hypothetical protein -
B2H83_RS01165 250203..251021 - 819 WP_129541688.1 DUF945 domain-containing protein -
B2H83_RS01170 251121..251354 - 234 WP_001213776.1 DUF905 family protein -
B2H83_RS01175 251472..251924 - 453 WP_000682723.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T73566 WP_000854730.1 NZ_CP019953:c247481-247104 [Escherichia coli M8]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T73566 NZ_CP019953:c247481-247104 [Escherichia coli M8]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT73566 WP_001285481.1 NZ_CP019953:c247902-247528 [Escherichia coli M8]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT73566 NZ_CP019953:c247902-247528 [Escherichia coli M8]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References