Detailed information of TA system TA073565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 309278..309994
• Replicon: chromosome
• Accession: NZ_CP019952
• Organism: Pseudomonas parafulva strain PRS09-11288

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: B2J77_RS01325
• Protein ID: WP_078477835.1
• Coordinates: 309812..309994 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8I0VNS3
• Locus tag: B2J77_RS01320
• Protein ID: WP_058638931.1
• Coordinates: 309278..309724 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2J77_RS01305	306474..306680	-	207	WP_078477832.1	DUF3079 domain-containing protein	-
B2J77_RS01310	307018..307830	+	813	WP_078477833.1	alpha/beta hydrolase	-
B2J77_RS01315	307823..308980	+	1158	WP_078477834.1	HAMP domain-containing histidine kinase	-
B2J77_RS01320	309278..309724	-	447	WP_058638931.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B2J77_RS01325	309812..309994	-	183	WP_078477835.1	type II toxin-antitoxin system HicA family toxin	Toxin
B2J77_RS01340	311117..311365	-	249	WP_078477836.1	hypothetical protein	-
B2J77_RS01345	311965..312255	+	291	WP_058639967.1	DUF3077 domain-containing protein	-
B2J77_RS01350	312682..313887	-	1206	WP_078477837.1	methyltransferase	-
B2J77_RS01355	314001..314690	-	690	WP_078477838.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6822.85 Da
• Isoelectric Point: 11.6882

>T73565 WP_078477835.1 NZ_CP019952:c309994-309812 [Pseudomonas parafulva]
VHSRDIIKRLKDDGWVRVKGTDGSHQQFKHPTRKGRVTVPHPSKDVAIGTLRNIWKQAGL

Nucleotide

• Length: 183 bp

>T73565 NZ_CP019952:c309994-309812 [Pseudomonas parafulva]
GTGCACAGCCGAGACATCATCAAGCGGCTAAAGGATGACGGTTGGGTCAGAGTTAAAGGGACTGATGGGAGCCACCAGCA
GTTCAAGCATCCAACCAGGAAGGGACGGGTTACGGTTCCTCATCCGAGTAAAGATGTGGCAATCGGGACGCTTCGAAACA
TCTGGAAGCAAGCCGGTCTCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16397.38 Da
• Isoelectric Point: 4.3924

>AT73565 WP_058638931.1 NZ_CP019952:c309724-309278 [Pseudomonas parafulva]
MKYPVVLHKDEDSDYGVTVPDVPGCFSAGETIDEALSEVQLALEMHFEGLVEDGEPLPQPQNIELHRKDPAYADGIWAFV
EFDVTPYYGKSVRFNASLPEGLLKRIDDRVKEDSSYASRSGFLAAAALHELAKNLNEGIDALKTRKTV

Nucleotide

• Length: 447 bp

>AT73565 NZ_CP019952:c309724-309278 [Pseudomonas parafulva]
ATGAAATACCCAGTTGTTTTGCACAAAGATGAAGATTCCGATTACGGAGTGACAGTTCCAGACGTACCGGGCTGCTTCTC
TGCTGGCGAAACGATCGATGAAGCATTGAGCGAAGTCCAACTTGCGTTAGAGATGCACTTTGAAGGTTTGGTAGAAGATG
GAGAGCCTCTGCCGCAACCTCAAAACATCGAACTTCATAGGAAGGACCCGGCTTATGCCGATGGCATCTGGGCTTTCGTA
GAATTCGACGTCACCCCCTACTATGGGAAGTCGGTTCGCTTTAACGCGTCTTTACCAGAAGGTCTCCTCAAACGCATAGA
TGATCGCGTGAAAGAAGACAGCTCTTATGCGTCCAGATCAGGATTTTTAGCCGCGGCGGCGCTGCACGAGCTGGCGAAAA
ACTTAAACGAAGGCATAGACGCTTTAAAAACCAGAAAGACAGTATGA