Detailed information of TA system TA073556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 32718..33379
• Replicon: chromosome
• Accession: NZ_CP019947
• Organism: Pseudomonas sp. CC6-YY-74

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B2D37_RS00160
• Protein ID: WP_079200832.1
• Coordinates: 32960..33379 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: B2D37_RS00155
• Protein ID: WP_079200831.1
• Coordinates: 32718..32963 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2D37_RS00130	28531..29064	-	534	WP_079200827.1	DUF3087 domain-containing protein	-
B2D37_RS00135	29318..29896	-	579	WP_079200828.1	S-(hydroxymethyl)glutathione synthase	-
B2D37_RS22920	30017..30244	-	228	WP_154067008.1	hypothetical protein	-
B2D37_RS00140	30593..30868	+	276	WP_079200829.1	hypothetical protein	-
B2D37_RS00145	31037..31696	+	660	Protein_19	hypothetical protein	-
B2D37_RS00150	31693..32682	+	990	WP_079200830.1	tyrosine-type recombinase/integrase	-
B2D37_RS00155	32718..32963	+	246	WP_079200831.1	plasmid stabilization protein	Antitoxin
B2D37_RS00160	32960..33379	+	420	WP_079200832.1	type II toxin-antitoxin system VapC family toxin	Toxin
B2D37_RS00165	33466..33792	+	327	WP_079200833.1	excalibur calcium-binding domain-containing protein	-
B2D37_RS00175	34048..35145	-	1098	WP_079200834.1	redox-regulated ATPase YchF	-
B2D37_RS00180	35166..35756	-	591	WP_079200835.1	aminoacyl-tRNA hydrolase	-
B2D37_RS00185	35826..36461	-	636	WP_079200836.1	50S ribosomal protein L25/general stress protein Ctc	-
B2D37_RS00190	36577..37518	-	942	WP_079204847.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15111.32 Da
• Isoelectric Point: 4.4185

>T73556 WP_079200832.1 NZ_CP019947:32960-33379 [Pseudomonas sp. CC6-YY-74]
MILLDTNVLSELMRAKPAAEVLAWVDAQPSSELFTSAITVAEILYGIARMPDGKRKDGLLELATLMFEEDFSGRILPFDT
EAAVHYAGLAAESEAKGRVVDMADGQIAAIAALHDARVATRNVRHFDYLGVPVINPWEA

Nucleotide

• Length: 420 bp

>T73556 NZ_CP019947:32960-33379 [Pseudomonas sp. CC6-YY-74]
ATGATCTTGCTGGATACCAACGTGCTTTCAGAGTTGATGCGGGCCAAGCCTGCTGCTGAAGTCTTGGCGTGGGTTGATGC
CCAGCCTTCAAGCGAGCTGTTCACCAGTGCCATCACCGTTGCTGAAATTCTCTACGGCATCGCCCGCATGCCTGACGGCA
AGCGTAAGGACGGTTTGTTAGAGCTGGCGACGCTGATGTTCGAAGAGGATTTTTCTGGCCGTATTCTTCCGTTTGATACA
GAAGCGGCTGTTCACTATGCCGGCTTAGCCGCTGAGAGTGAGGCGAAAGGGAGGGTCGTGGATATGGCCGATGGGCAGAT
CGCTGCCATTGCCGCTTTGCATGATGCGCGAGTCGCTACGCGCAATGTTCGGCACTTTGACTACCTGGGCGTTCCCGTTA
TCAATCCTTGGGAGGCTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8806.04 Da
• Isoelectric Point: 10.1758

>AT73556 WP_079200831.1 NZ_CP019947:32718-32963 [Pseudomonas sp. CC6-YY-74]
MASITIRNLDDDIKAQLRVVAASHGRSMEEEVRVIIRHALFKSEKSGGLGSRINARFAAVGGVDLEVPARKDKARAASFD
E

Nucleotide

• Length: 246 bp

>AT73556 NZ_CP019947:32718-32963 [Pseudomonas sp. CC6-YY-74]
ATGGCCAGCATCACTATTCGAAACCTAGACGACGACATCAAGGCTCAACTGCGTGTTGTTGCCGCAAGCCATGGCCGTTC
GATGGAAGAAGAGGTCAGGGTCATTATTCGCCATGCGCTGTTTAAGTCAGAAAAAAGCGGTGGCTTGGGTAGTCGCATCA
ATGCTCGATTCGCTGCCGTTGGTGGGGTGGATCTCGAAGTGCCTGCTCGCAAGGACAAGGCCCGTGCCGCGAGCTTTGAC
GAATGA