Detailed information of TA system TA073548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2210516..2211045
• Replicon: chromosome
• Accession: NZ_CP019945
• Organism: Staphylococcus aureus strain BA01611

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AOZ05_RS11645
• Protein ID: WP_000621175.1
• Coordinates: 2210516..2210878 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AOZ05_RS11650
• Protein ID: WP_000948331.1
• Coordinates: 2210875..2211045 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOZ05_RS11615	2206138..2207310	+	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
AOZ05_RS11620	2207494..2208264	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
AOZ05_RS11625	2208239..2208718	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AOZ05_RS11630	2208720..2209046	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AOZ05_RS11635	2209165..2210166	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AOZ05_RS11645	2210516..2210878	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AOZ05_RS11650	2210875..2211045	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AOZ05_RS11655	2211130..2212278	-	1149	WP_001281154.1	alanine racemase	-
AOZ05_RS11660	2212344..2212703	-	360	WP_000581197.1	holo-ACP synthase	-
AOZ05_RS11665	2212707..2213198	-	492	WP_072353916.1	PH domain-containing protein	-
AOZ05_RS11670	2213185..2214768	-	1584	WP_001294626.1	PH domain-containing protein	-
AOZ05_RS11675	2214761..2215240	-	480	WP_031590204.1	hypothetical protein	-
AOZ05_RS11680	2215449..2216009	-	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2206138..2207310	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T73548 WP_000621175.1 NZ_CP019945:c2210878-2210516 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T73548 NZ_CP019945:c2210878-2210516 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT73548 WP_000948331.1 NZ_CP019945:c2211045-2210875 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT73548 NZ_CP019945:c2211045-2210875 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1