Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4898213..4899045 Replicon chromosome
Accession NZ_CP019944
Organism Escherichia coli strain DSM 103246

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag Eco28_RS25095 Protein ID WP_000854753.1
Coordinates 4898213..4898587 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag Eco28_RS25100 Protein ID WP_001278232.1
Coordinates 4898677..4899045 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco28_RS25070 4894566..4896104 - 1539 WP_001187177.1 lysine decarboxylation/transport transcriptional activator CadC -
Eco28_RS25080 4896853..4897422 - 570 WP_001290247.1 DUF4942 domain-containing protein -
Eco28_RS25085 4897519..4897716 - 198 WP_000839281.1 DUF957 domain-containing protein -
Eco28_RS25090 4897728..4898216 - 489 WP_000777541.1 hypothetical protein -
Eco28_RS25095 4898213..4898587 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
Eco28_RS25100 4898677..4899045 - 369 WP_001278232.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
Eco28_RS25110 4899208..4899429 - 222 WP_000692303.1 DUF987 domain-containing protein -
Eco28_RS25115 4899492..4899968 - 477 WP_001186710.1 RadC family protein -
Eco28_RS25120 4899980..4900459 - 480 WP_000860065.1 antirestriction protein -
Eco28_RS25125 4900541..4901359 - 819 WP_080030379.1 DUF945 domain-containing protein -
Eco28_RS25130 4901458..4901691 - 234 WP_001278282.1 DUF905 family protein -
Eco28_RS25135 4901697..4902374 - 678 WP_001097364.1 hypothetical protein -
Eco28_RS25140 4902493..4903377 - 885 WP_032216515.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T73541 WP_000854753.1 NZ_CP019944:c4898587-4898213 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T73541 NZ_CP019944:c4898587-4898213 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT73541 WP_001278232.1 NZ_CP019944:c4899045-4898677 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT73541 NZ_CP019944:c4899045-4898677 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References