Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4673702..4674297 Replicon chromosome
Accession NZ_CP019944
Organism Escherichia coli strain DSM 103246

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag Eco28_RS23780 Protein ID WP_000239579.1
Coordinates 4673702..4674052 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag Eco28_RS23785 Protein ID WP_001223208.1
Coordinates 4674046..4674297 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco28_RS23760 4669148..4670170 - 1023 WP_001362056.1 ABC transporter permease -
Eco28_RS23765 4670184..4671686 - 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
Eco28_RS23770 4671826..4672782 - 957 WP_000265942.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
Eco28_RS23775 4673092..4673622 + 531 WP_000055072.1 inorganic diphosphatase -
Eco28_RS23780 4673702..4674052 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
Eco28_RS23785 4674046..4674297 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
Eco28_RS23790 4674588..4675583 - 996 WP_001372721.1 tyrosine-type recombinase/integrase -
Eco28_RS23795 4675650..4675949 - 300 WP_021580386.1 helix-turn-helix transcriptional regulator -
Eco28_RS23800 4676058..4676483 + 426 WP_001158283.1 regulatory protein -
Eco28_RS23805 4676521..4676871 + 351 WP_000664580.1 DUF4761 family protein -
Eco28_RS23810 4676882..4677160 + 279 WP_000159895.1 hypothetical protein -
Eco28_RS23815 4677172..4677414 + 243 WP_000514277.1 DUF4754 family protein -
Eco28_RS28295 4677411..4677524 + 114 WP_000021656.1 hypothetical protein -
Eco28_RS23820 4677611..4677814 + 204 WP_001560998.1 hypothetical protein -
Eco28_RS23825 4677811..4678056 + 246 WP_000153676.1 winged helix-turn-helix domain-containing protein -
Eco28_RS23830 4678053..4678367 + 315 WP_046788693.1 ead/Ea22-like family protein -
Eco28_RS23835 4678364..4678567 + 204 WP_001036809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4668166..4710006 41840


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T73538 WP_000239579.1 NZ_CP019944:c4674052-4673702 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T73538 NZ_CP019944:c4674052-4673702 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT73538 WP_001223208.1 NZ_CP019944:c4674297-4674046 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT73538 NZ_CP019944:c4674297-4674046 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References