Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4452681..4453231 Replicon chromosome
Accession NZ_CP019944
Organism Escherichia coli strain DSM 103246

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag Eco28_RS22720 Protein ID WP_001160974.1
Coordinates 4452681..4452995 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag Eco28_RS22725 Protein ID WP_000125566.1
Coordinates 4452998..4453231 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco28_RS22695 4448356..4449642 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
Eco28_RS22700 4449642..4450631 + 990 WP_000241272.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
Eco28_RS22705 4450628..4451449 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
Eco28_RS22710 4451452..4451829 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
Eco28_RS22715 4451836..4452684 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
Eco28_RS22720 4452681..4452995 - 315 WP_001160974.1 CcdB family protein Toxin
Eco28_RS22725 4452998..4453231 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
Eco28_RS22730 4453317..4453796 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
Eco28_RS22735 4453988..4455850 - 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
Eco28_RS22740 4455843..4456373 - 531 WP_000600729.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
Eco28_RS22745 4456484..4457815 - 1332 WP_001183198.1 MFS transporter -
Eco28_RS22750 4457873..4458160 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T73537 WP_001160974.1 NZ_CP019944:c4452995-4452681 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T73537 NZ_CP019944:c4452995-4452681 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT73537 WP_000125566.1 NZ_CP019944:c4453231-4452998 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT73537 NZ_CP019944:c4453231-4452998 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References