Detailed information of TA system TA073535

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4131604..4132439
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: Eco28_RS21160
• Protein ID: WP_000854821.1
• Coordinates: 4131604..4131981 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0D404
• Locus tag: Eco28_RS21165
• Protein ID: WP_024214935.1
• Coordinates: 4132071..4132439 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS21135	4127156..4128112	+	957	WP_000121355.1	molybdenum cofactor insertion chaperone PaoD	-
Eco28_RS21140	4128163..4128501	+	339	Protein_3904	LysR family transcriptional regulator	-
Eco28_RS21145	4128840..4130159	+	1320	WP_000144688.1	site-specific integrase	-
Eco28_RS21150	4130252..4131100	-	849	WP_001280470.1	DUF4942 domain-containing protein	-
Eco28_RS28235	4131185..4131382	-	198	WP_089075429.1	DUF957 domain-containing protein	-
Eco28_RS28240	4131410..4131607	-	198	Protein_3908	hypothetical protein	-
Eco28_RS21160	4131604..4131981	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
Eco28_RS21165	4132071..4132439	-	369	WP_024214935.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
Eco28_RS21170	4132519..4132740	-	222	WP_000692350.1	DUF987 domain-containing protein	-
Eco28_RS21175	4132827..4133303	-	477	WP_001186745.1	RadC family protein	-
Eco28_RS21180	4133319..4133804	-	486	WP_024214936.1	antirestriction protein	-
Eco28_RS21185	4133896..4134714	-	819	WP_001234397.1	DUF945 domain-containing protein	-
Eco28_RS21190	4134804..4135037	-	234	WP_001117566.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4086675..4164520	77845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T73535 WP_000854821.1 NZ_CP019944:c4131981-4131604 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T73535 NZ_CP019944:c4131981-4131604 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13605.41 Da
• Isoelectric Point: 6.4659

>AT73535 WP_024214935.1 NZ_CP019944:c4132439-4132071 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73535 NZ_CP019944:c4132439-4132071 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCAACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54

Antitoxin

• AlphaFold DB: A0A1M0D404