Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3915385..3916003 Replicon chromosome
Accession NZ_CP019944
Organism Escherichia coli strain DSM 103246

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag Eco28_RS20090 Protein ID WP_001291435.1
Coordinates 3915785..3916003 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag Eco28_RS20085 Protein ID WP_000344800.1
Coordinates 3915385..3915759 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco28_RS20075 3910474..3911667 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
Eco28_RS20080 3911690..3914839 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
Eco28_RS20085 3915385..3915759 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
Eco28_RS20090 3915785..3916003 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
Eco28_RS20095 3916175..3916726 + 552 WP_080030289.1 maltose O-acetyltransferase -
Eco28_RS20100 3916842..3917312 + 471 WP_000136192.1 YlaC family protein -
Eco28_RS20105 3917476..3919026 + 1551 WP_072686422.1 cyclic-guanylate-specific phosphodiesterase PdeB -
Eco28_RS20110 3919068..3919421 - 354 WP_080030290.1 DUF1428 family protein -
Eco28_RS20120 3919800..3920111 + 312 WP_172951475.1 MGMT family protein -
Eco28_RS20125 3920142..3920714 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73534 WP_001291435.1 NZ_CP019944:3915785-3916003 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T73534 NZ_CP019944:3915785-3916003 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT73534 WP_000344800.1 NZ_CP019944:3915385-3915759 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT73534 NZ_CP019944:3915385-3915759 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References