Detailed information of TA system TA073533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3496426..3497131
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A246P0D5
• Locus tag: Eco28_RS18090
• Protein ID: WP_000539522.1
• Coordinates: 3496426..3496812 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: Eco28_RS18095
• Protein ID: WP_001280945.1
• Coordinates: 3496802..3497131 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS18070	3492430..3493056	+	627	WP_001295292.1	glutathione S-transferase GstB	-
Eco28_RS18075	3493053..3494168	-	1116	WP_021577174.1	aldose sugar dehydrogenase YliI	-
Eco28_RS18080	3494279..3494662	-	384	WP_000497137.1	biofilm formation regulator BssR	-
Eco28_RS18085	3494875..3496200	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
Eco28_RS18090	3496426..3496812	+	387	WP_000539522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Eco28_RS18095	3496802..3497131	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
Eco28_RS18100	3497201..3498529	-	1329	WP_000086864.1	GGDEF domain-containing protein	-
Eco28_RS18105	3498537..3499661	-	1125	Protein_3355	EAL domain-containing protein	-
Eco28_RS18110	3499765..3500973	+	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-
Eco28_RS18115	3500952..3501527	-	576	WP_074410597.1	CHASE9 sensor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3499765..3500973	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14292.51 Da
• Isoelectric Point: 10.2120

>T73533 WP_000539522.1 NZ_CP019944:3496426-3496812 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIKDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T73533 NZ_CP019944:3496426-3496812 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAAAGGATTTGATCGAAACCGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT73533 WP_001280945.1 NZ_CP019944:3496802-3497131 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT73533 NZ_CP019944:3496802-3497131 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A246P0D5