Detailed information of TA system TA073532

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3402376..3403211
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8H9T613
• Locus tag: Eco28_RS17600
• Protein ID: WP_069723388.1
• Coordinates: 3402376..3402753 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3Z2YIX3
• Locus tag: Eco28_RS17605
• Protein ID: WP_032178229.1
• Coordinates: 3402843..3403211 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS17565	3399057..3399761	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
Eco28_RS17575	3400046..3400264	+	219	WP_001040187.1	translation initiation factor IF-1	-
Eco28_RS17585	3400755..3401597	-	843	WP_080030236.1	DUF4942 domain-containing protein	-
Eco28_RS17590	3401682..3401879	-	198	WP_001374283.1	DUF957 domain-containing protein	-
Eco28_RS17595	3401891..3402379	-	489	WP_058101247.1	hypothetical protein	-
Eco28_RS17600	3402376..3402753	-	378	WP_069723388.1	TA system toxin CbtA family protein	Toxin
Eco28_RS17605	3402843..3403211	-	369	WP_032178229.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
Eco28_RS17610	3403261..3403905	-	645	WP_000086748.1	hypothetical protein	-
Eco28_RS17615	3403924..3404145	-	222	WP_069723389.1	DUF987 domain-containing protein	-
Eco28_RS17620	3404232..3404630	-	399	WP_069723390.1	RadC family protein	-
Eco28_RS17625	3404646..3405131	-	486	WP_069723391.1	antirestriction protein	-
Eco28_RS17630	3405223..3406041	-	819	WP_024220054.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.05 Da
• Isoelectric Point: 9.1376

>T73532 WP_069723388.1 NZ_CP019944:c3402753-3402376 [Escherichia coli]
MKTLPVLPGQAAGSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDKPGF
NACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHSEAKR

Nucleotide

• Length: 378 bp

>T73532 NZ_CP019944:c3402753-3402376 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTTAC
CAGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACGCCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACAAGCCGGGATTC
AACGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTGAACAATATCACCCGGGGTAAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13823.58 Da
• Isoelectric Point: 6.3177

>AT73532 WP_032178229.1 NZ_CP019944:c3403211-3402843 [Escherichia coli]
VSDTLHETNYPDDNNDRSWWGLPCTVTPCFRARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73532 NZ_CP019944:c3403211-3402843 [Escherichia coli]
GTGTCAGACACACTCCATGAAACAAATTATCCCGACGACAACAACGACCGCTCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCAGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGTCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3Z2YIX3