Detailed information of TA system TA073531

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3133988..3134823
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: Eco28_RS16335
• Protein ID: WP_000854768.1
• Coordinates: 3133988..3134365 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: Eco28_RS16340
• Protein ID: WP_024179191.1
• Coordinates: 3134455..3134823 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS16300	3129576..3130130	-	555	WP_001001918.1	molecular chaperone YcdY	-
Eco28_RS16305	3130154..3130891	-	738	WP_000283664.1	zinc-binding phosphatase	-
Eco28_RS16310	3130946..3131884	-	939	WP_000351322.1	glyoxylate/hydroxypyruvate reductase GhrA	-
Eco28_RS16320	3132355..3133197	-	843	WP_072693077.1	DUF4942 domain-containing protein	-
Eco28_RS16325	3133294..3133491	-	198	WP_000839234.1	DUF957 domain-containing protein	-
Eco28_RS16330	3133503..3133991	-	489	WP_000761708.1	hypothetical protein	-
Eco28_RS16335	3133988..3134365	-	378	WP_000854768.1	TA system toxin CbtA family protein	Toxin
Eco28_RS16340	3134455..3134823	-	369	WP_024179191.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
Eco28_RS16345	3134873..3135517	-	645	WP_000086766.1	hypothetical protein	-
Eco28_RS16350	3135536..3135757	-	222	WP_000692290.1	DUF987 domain-containing protein	-
Eco28_RS16355	3135826..3136302	-	477	WP_001470113.1	RadC family protein	-
Eco28_RS16360	3136318..3136803	-	486	WP_000206651.1	antirestriction protein	-
Eco28_RS16365	3136896..3137714	-	819	WP_080030209.1	DUF945 domain-containing protein	-
Eco28_RS16380	3138054..3139124	-	1071	WP_029399299.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14148.25 Da
• Isoelectric Point: 7.7294

>T73531 WP_000854768.1 NZ_CP019944:c3134365-3133988 [Escherichia coli]
MKTLPDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
GACTCSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T73531 NZ_CP019944:c3134365-3133988 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGTATCGCGCTGCCCGTCTCCCGTAACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTCGTTGAAAAATACGCCCTGGTACGTACCGACCAGCCGGGATTC
GGCGCCTGTACCTGTTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13734.44 Da
• Isoelectric Point: 5.4970

>AT73531 WP_024179191.1 NZ_CP019944:c3134823-3134455 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73531 NZ_CP019944:c3134823-3134455 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCTTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCAGACCGCGCCGGCATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACTCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA