Detailed information of TA system TA073530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2670867..2671505
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A246NT23
• Locus tag: Eco28_RS13820
• Protein ID: WP_021577430.1
• Coordinates: 2671329..2671505 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: Eco28_RS13815
• Protein ID: WP_001270286.1
• Coordinates: 2670867..2671283 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS13795	2666019..2666960	-	942	WP_021577433.1	ABC transporter permease	-
Eco28_RS13800	2666961..2667974	-	1014	WP_021577432.1	ABC transporter ATP-binding protein	-
Eco28_RS13805	2667992..2669137	-	1146	WP_021577431.1	ABC transporter substrate-binding protein	-
Eco28_RS13810	2669382..2670788	-	1407	WP_000760665.1	PLP-dependent aminotransferase family protein	-
Eco28_RS13815	2670867..2671283	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
Eco28_RS13820	2671329..2671505	-	177	WP_021577430.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
Eco28_RS13825	2671727..2671957	+	231	WP_000494244.1	YncJ family protein	-
Eco28_RS13830	2672049..2674010	-	1962	WP_021577429.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
Eco28_RS13835	2674083..2674619	-	537	WP_021577428.1	DNA-binding transcriptional regulator SutR	-
Eco28_RS13840	2674672..2675886	+	1215	WP_071780838.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2675926..2677074	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6692.77 Da
• Isoelectric Point: 10.9223

>T73530 WP_021577430.1 NZ_CP019944:c2671505-2671329 [Escherichia coli]
VKQSEFRRWLESQGVDVANVSNHLKLGFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T73530 NZ_CP019944:c2671505-2671329 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGTCAGCAACCATTTGAAACTTGG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT73530 WP_001270286.1 NZ_CP019944:c2671283-2670867 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT73530 NZ_CP019944:c2671283-2670867 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A246NT23