Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2006757..2007588 Replicon chromosome
Accession NZ_CP019944
Organism Escherichia coli strain DSM 103246

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag Eco28_RS10220 Protein ID WP_000854814.1
Coordinates 2006757..2007131 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag Eco28_RS10225 Protein ID WP_001285585.1
Coordinates 2007220..2007588 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco28_RS10185 2002153..2003319 + 1167 WP_001310935.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
Eco28_RS10190 2003438..2003911 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
Eco28_RS10195 2004109..2005167 + 1059 WP_001200891.1 FUSC family protein -
Eco28_RS10200 2005339..2005668 + 330 WP_000450409.1 DUF496 family protein -
Eco28_RS10205 2005769..2005951 - 183 WP_001016348.1 hypothetical protein -
Eco28_RS10210 2006023..2006151 + 129 Protein_1894 transposase domain-containing protein -
Eco28_RS28065 2006440..2006553 - 114 WP_001161660.1 DUF957 domain-containing protein -
Eco28_RS10215 2006566..2006760 - 195 WP_000988600.1 hypothetical protein -
Eco28_RS10220 2006757..2007131 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
Eco28_RS10225 2007220..2007588 - 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
Eco28_RS10230 2007662..2007883 - 222 WP_000692323.1 DUF987 domain-containing protein -
Eco28_RS10235 2007946..2008422 - 477 WP_001186773.1 RadC family protein -
Eco28_RS10240 2008438..2008917 - 480 WP_000860076.1 antirestriction protein -
Eco28_RS10245 2008999..2009820 - 822 WP_001234530.1 DUF945 domain-containing protein -
Eco28_RS10255 2010041..2010451 - 411 WP_000846713.1 hypothetical protein -
Eco28_RS10260 2010467..2011150 - 684 WP_080030092.1 hypothetical protein -
Eco28_RS10265 2011286..2012356 - 1071 WP_000102643.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T73519 WP_000854814.1 NZ_CP019944:c2007131-2006757 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T73519 NZ_CP019944:c2007131-2006757 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT73519 WP_001285585.1 NZ_CP019944:c2007588-2007220 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT73519 NZ_CP019944:c2007588-2007220 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References