Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1494861..1495486 Replicon chromosome
Accession NZ_CP019944
Organism Escherichia coli strain DSM 103246

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag Eco28_RS07755 Protein ID WP_000911329.1
Coordinates 1495088..1495486 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag Eco28_RS07750 Protein ID WP_000450524.1
Coordinates 1494861..1495088 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco28_RS07725 1490663..1491133 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
Eco28_RS07730 1491133..1491705 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
Eco28_RS07735 1491851..1492729 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
Eco28_RS07740 1492746..1493780 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
Eco28_RS07745 1493993..1494706 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
Eco28_RS07750 1494861..1495088 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
Eco28_RS07755 1495088..1495486 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
Eco28_RS07760 1495633..1496496 + 864 WP_001267498.1 neutral zinc metallopeptidase -
Eco28_RS07765 1496511..1498526 + 2016 WP_080030036.1 tRNA cytosine(34) acetyltransferase TmcA -
Eco28_RS07770 1498600..1499298 + 699 WP_000679812.1 esterase -
Eco28_RS07775 1499379..1499579 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T73517 WP_000911329.1 NZ_CP019944:1495088-1495486 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T73517 NZ_CP019944:1495088-1495486 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT73517 WP_000450524.1 NZ_CP019944:1494861-1495088 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT73517 NZ_CP019944:1494861-1495088 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTACCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References