Detailed information of TA system TA073514

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 840660..841495
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: Eco28_RS04260
• Protein ID: WP_000854821.1
• Coordinates: 840660..841037 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: Eco28_RS04265
• Protein ID: WP_080029956.1
• Coordinates: 841127..841495 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS04240	836690..837571	+	882	WP_021577941.1	integrase domain-containing protein	-
Eco28_RS04245	837753..839020	-	1268	Protein_776	integrase arm-type DNA-binding domain-containing protein	-
Eco28_RS04250	839314..840156	-	843	WP_080029955.1	DUF4942 domain-containing protein	-
Eco28_RS27955	840241..840438	-	198	WP_087904847.1	DUF957 domain-containing protein	-
Eco28_RS27960	840514..840663	-	150	Protein_779	hypothetical protein	-
Eco28_RS04260	840660..841037	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
Eco28_RS04265	841127..841495	-	369	WP_080029956.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
Eco28_RS04270	841575..841796	-	222	WP_000692350.1	DUF987 domain-containing protein	-
Eco28_RS04275	841883..842359	-	477	WP_001186780.1	RadC family protein	-
Eco28_RS04280	842375..842860	-	486	WP_000213716.1	antirestriction protein	-
Eco28_RS04285	842952..843770	-	819	WP_053271315.1	DUF945 domain-containing protein	-
Eco28_RS04290	843860..844069	-	210	WP_032150870.1	DUF905 family protein	-
Eco28_RS04295	844099..844776	-	678	WP_001097301.1	hypothetical protein	-
Eco28_RS04300	844924..845604	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	837753..867283	29530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T73514 WP_000854821.1 NZ_CP019944:c841037-840660 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T73514 NZ_CP019944:c841037-840660 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13572.36 Da
• Isoelectric Point: 6.0618

>AT73514 WP_080029956.1 NZ_CP019944:c841495-841127 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73514 NZ_CP019944:c841495-841127 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54