Detailed information of TA system TA073511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 337059..337859
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: Eco28_RS01625
• Protein ID: WP_000342448.1
• Coordinates: 337332..337859 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: U9Y285
• Locus tag: Eco28_RS01620
• Protein ID: WP_001277109.1
• Coordinates: 337059..337325 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS01600	332717..333385	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
Eco28_RS01605	333378..334436	+	1059	WP_001042006.1	permease-like cell division protein FtsX	-
Eco28_RS01610	334681..335535	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
Eco28_RS01615	335806..336909	+	1104	WP_001022009.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
Eco28_RS01620	337059..337325	+	267	WP_001277109.1	DUF1778 domain-containing protein	Antitoxin
Eco28_RS01625	337332..337859	+	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
Eco28_RS01630	337856..338239	-	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
Eco28_RS01640	338663..339772	+	1110	WP_080029919.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
Eco28_RS01645	339820..340746	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
Eco28_RS01650	340743..342020	+	1278	WP_042063469.1	branched chain amino acid ABC transporter permease LivM	-
Eco28_RS01655	342017..342784	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T73511 WP_000342448.1 NZ_CP019944:337332-337859 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T73511 NZ_CP019944:337332-337859 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9938.37 Da
• Isoelectric Point: 10.0863

>AT73511 WP_001277109.1 NZ_CP019944:337059-337325 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Nucleotide

• Length: 267 bp

>AT73511 NZ_CP019944:337059-337325 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• AlphaFold DB: A0A7U9LUN0