Detailed information of TA system TA073510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 299982..300613
• Replicon: chromosome
• Accession: NZ_CP019944
• Organism: Escherichia coli strain DSM 103246

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: Eco28_RS01405
• Protein ID: WP_072686259.1
• Coordinates: 299982..300257 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q31VE4
• Locus tag: Eco28_RS01410
• Protein ID: WP_000593554.1
• Coordinates: 300254..300613 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco28_RS01390	294986..296053	+	1068	WP_000361517.1	HlyD family secretion protein	-
Eco28_RS01395	296050..298785	+	2736	WP_072686258.1	ribosome-associated ATPase/putative transporter RbbA	-
Eco28_RS01400	298785..299909	+	1125	WP_001314210.1	ABC transporter permease	-
Eco28_RS01405	299982..300257	+	276	WP_072686259.1	type II toxin-antitoxin system HicA family toxin	Toxin
Eco28_RS01410	300254..300613	+	360	WP_000593554.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Eco28_RS01415	300733..301134	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
Eco28_RS01420	301140..301946	-	807	WP_032288306.1	nickel import ATP-binding protein NikE	-
Eco28_RS01425	301943..302707	-	765	WP_047644476.1	nickel import ATP-binding protein NikD	-
Eco28_RS01430	302707..303540	-	834	WP_072686260.1	nickel ABC transporter permease subunit NikC	-
Eco28_RS01435	303537..304481	-	945	WP_000947082.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	276913..300613	23700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10166.87 Da
• Isoelectric Point: 10.7076

>T73510 WP_072686259.1 NZ_CP019944:299982-300257 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T73510 NZ_CP019944:299982-300257 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13359.25 Da
• Isoelectric Point: 4.8838

>AT73510 WP_000593554.1 NZ_CP019944:300254-300613 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT73510 NZ_CP019944:300254-300613 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A6H2GM94