Detailed information of TA system TA073487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 32043..32590
• Replicon: plasmid unnamed4
• Accession: NZ_CP019910
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: B1200_RS29030
• Protein ID: WP_000969983.1
• Coordinates: 32043..32324 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5JBQ7
• Locus tag: B1200_RS29035
• Protein ID: WP_000079943.1
• Coordinates: 32321..32590 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS29000	29617..30054	-	438	Protein_41	recombinase family protein	-
B1200_RS29010	30108..30812	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
B1200_RS29015	30877..31170	+	294	Protein_43	mobilization protein MobA	-
B1200_RS29020	31245..31949	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
B1200_RS29030	32043..32324	-	282	WP_000969983.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B1200_RS29035	32321..32590	-	270	WP_000079943.1	hypothetical protein	Antitoxin
B1200_RS29045	33503..34360	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
B1200_RS29050	34353..34427	-	75	WP_001365705.1	RepA leader peptide Tap	-
B1200_RS29060	34665..34919	-	255	WP_000083830.1	replication regulatory protein RepA	-
B1200_RS29065	35159..35749	-	591	WP_000766807.1	DUF2726 domain-containing protein	-
B1200_RS29070	35787..35996	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
B1200_RS29075	36042..36503	-	462	WP_001233838.1	thermonuclease family protein	-
B1200_RS29080	36748..36960	-	213	WP_001309245.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68786	68786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10874.52 Da
• Isoelectric Point: 7.3987

>T73487 WP_000969983.1 NZ_CP019910:c32324-32043 [Escherichia coli]
MMEIFWTMLASQDRKHIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T73487 NZ_CP019910:c32324-32043 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCATATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10272.57 Da
• Isoelectric Point: 7.2057

>AT73487 WP_000079943.1 NZ_CP019910:c32590-32321 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLSHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT73487 NZ_CP019910:c32590-32321 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGCCAGCAACTTGAGCGCGGTGATGTGCTTTCCCATGACATGGTCGAATCCTCAGCTACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A622US83