Detailed information of TA system TA073483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 50139..50674
• Replicon: plasmid unnamed3
• Accession: NZ_CP019909
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: B1200_RS28545
• Protein ID: WP_000222760.1
• Coordinates: 50139..50426 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: B1200_RS28550
• Protein ID: WP_001132900.1
• Coordinates: 50423..50674 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS28510	45722..46943	-	1222	Protein_47	ISL3 family transposase	-
B1200_RS28515	47043..47407	+	365	Protein_48	IS1 family transposase	-
B1200_RS28520	47462..48244	-	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
B1200_RS28525	48241..49263	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
B1200_RS28535	49352..49699	+	348	Protein_51	IS1-like element IS1A family transposase	-
B1200_RS28545	50139..50426	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B1200_RS28550	50423..50674	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B1200_RS28555	51637..52494	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
B1200_RS28560	52487..52969	-	483	WP_001273588.1	hypothetical protein	-
B1200_RS28565	52962..53036	-	75	WP_001442103.1	RepA leader peptide Tap	-
B1200_RS28575	53268..53525	-	258	WP_000083845.1	replication regulatory protein RepA	-
B1200_RS28580	53809..54015	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
-	54002..54063	+	62	NuclAT_1	-	-
-	54002..54063	+	62	NuclAT_1	-	-
-	54002..54063	+	62	NuclAT_1	-	-
-	54002..54063	+	62	NuclAT_1	-	-
B1200_RS30395	54319..54393	-	75	Protein_59	endonuclease	-
B1200_RS28590	54639..54851	-	213	WP_001299730.1	ANR family transcriptional regulator	-
B1200_RS28595	54987..55547	-	561	WP_000139315.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..85009	85009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T73483 WP_000222760.1 NZ_CP019909:c50426-50139 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T73483 NZ_CP019909:c50426-50139 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT73483 WP_001132900.1 NZ_CP019909:c50674-50423 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT73483 NZ_CP019909:c50674-50423 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2