Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 8568..9160 Replicon plasmid pMCR1-NY
Accession NZ_CP019908
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag B1200_RS28040 Protein ID WP_000520549.1
Coordinates 8568..8816 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag B1200_RS28045 Protein ID WP_000681613.1
Coordinates 8813..9160 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS28005 5445..5960 + 516 WP_001025395.1 J domain-containing protein -
B1200_RS28010 6065..6607 + 543 WP_001083906.1 hypothetical protein -
B1200_RS28015 6604..6855 + 252 WP_001230707.1 hypothetical protein -
B1200_RS28020 6842..7069 + 228 WP_000801440.1 hypothetical protein -
B1200_RS28030 7523..7822 + 300 WP_000650309.1 hypothetical protein -
B1200_RS28035 7913..8263 + 351 WP_000854259.1 hypothetical protein -
B1200_RS28040 8568..8816 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
B1200_RS28045 8813..9160 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B1200_RS28055 9521..10024 + 504 WP_000781813.1 hypothetical protein -
B1200_RS28060 10028..11257 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
B1200_RS28065 11334..11627 + 294 WP_060598542.1 LuxR family transcriptional regulator -
B1200_RS28070 11624..11839 + 216 WP_000760375.1 EexN family lipoprotein -
B1200_RS28075 12195..12815 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
B1200_RS28080 12883..13155 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33304 33304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T73478 WP_000520549.1 NZ_CP019908:8568-8816 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T73478 NZ_CP019908:8568-8816 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT73478 WP_000681613.1 NZ_CP019908:8813-9160 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT73478 NZ_CP019908:8813-9160 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References