73477

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6604..7069 Replicon plasmid pMCR1-NY
Accession NZ_CP019908
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag B1200_RS28015 Protein ID WP_001230707.1
Coordinates 6604..6855 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag B1200_RS28020 Protein ID WP_000801440.1
Coordinates 6842..7069 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS27985 2085..2612 - 528 WP_000577043.1 DUF2726 domain-containing protein -
B1200_RS27990 2747..3151 - 405 WP_012421203.1 hypothetical protein -
B1200_RS27995 3164..4030 - 867 WP_001353743.1 replication initiation protein -
B1200_RS28005 5445..5960 + 516 WP_001025395.1 J domain-containing protein -
B1200_RS28010 6065..6607 + 543 WP_001083906.1 hypothetical protein -
B1200_RS28015 6604..6855 + 252 WP_001230707.1 hypothetical protein Toxin
B1200_RS28020 6842..7069 + 228 WP_000801440.1 hypothetical protein Antitoxin
B1200_RS28030 7523..7822 + 300 WP_000650309.1 hypothetical protein -
B1200_RS28035 7913..8263 + 351 WP_000854259.1 hypothetical protein -
B1200_RS28040 8568..8816 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
B1200_RS28045 8813..9160 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
B1200_RS28055 9521..10024 + 504 WP_000781813.1 hypothetical protein -
B1200_RS28060 10028..11257 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
B1200_RS28065 11334..11627 + 294 WP_060598542.1 LuxR family transcriptional regulator -
B1200_RS28070 11624..11839 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33304 33304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(12-128)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T73477 WP_001230707.1 NZ_CP019908:6604-6855 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T73477 NZ_CP019908:6604-6855 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT73477 WP_000801440.1 NZ_CP019908:6842-7069 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT73477 NZ_CP019908:6842-7069 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References