Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 10238..10863 Replicon plasmid unnamed5
Accession NZ_CP019906
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag B1200_RS27710 Protein ID WP_000911329.1
Coordinates 10465..10863 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag B1200_RS27705 Protein ID WP_000450524.1
Coordinates 10238..10465 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS27680 6039..6509 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
B1200_RS27685 6509..7081 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
B1200_RS27690 7227..8105 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
B1200_RS27695 8122..9156 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
B1200_RS27700 9369..10082 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
B1200_RS27705 10238..10465 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
B1200_RS27710 10465..10863 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
B1200_RS27715 11010..11873 + 864 WP_001267498.1 neutral zinc metallopeptidase -
B1200_RS27720 11888..13867 + 1980 WP_078081121.1 tRNA cytosine(34) acetyltransferase TmcA -
B1200_RS27730 13971..15179 + 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
B1200_RS27735 15193..15684 + 492 Protein_16 lipopolysaccharide heptosyltransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..29168 29168


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T73476 WP_000911329.1 NZ_CP019906:10465-10863 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T73476 NZ_CP019906:10465-10863 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT73476 WP_000450524.1 NZ_CP019906:10238-10465 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT73476 NZ_CP019906:10238-10465 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References