Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20284..20809 Replicon plasmid unnamed6
Accession NZ_CP019905
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag B1200_RS26880 Protein ID WP_001159871.1
Coordinates 20284..20589 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag B1200_RS26885 Protein ID WP_000813630.1
Coordinates 20591..20809 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS26855 16456..16872 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
B1200_RS26860 16865..17845 - 981 WP_000688514.1 plasmid segregation protein ParM -
B1200_RS26865 18259..18567 - 309 WP_000030199.1 hypothetical protein -
B1200_RS26870 18654..19298 - 645 WP_001144036.1 ParA family protein -
B1200_RS26875 19477..20283 - 807 WP_078081109.1 site-specific integrase -
B1200_RS26880 20284..20589 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
B1200_RS26885 20591..20809 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
B1200_RS26900 21387..22475 + 1089 WP_000952217.1 transcriptional repressor PifC -
B1200_RS26905 22477..24702 + 2226 WP_000698737.1 phage T7 exclusion protein -
B1200_RS26910 24752..25651 - 900 WP_000963206.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-12 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / aph(3')-IIa - 1..142304 142304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T73470 WP_001159871.1 NZ_CP019905:c20589-20284 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T73470 NZ_CP019905:c20589-20284 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT73470 WP_000813630.1 NZ_CP019905:c20809-20591 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT73470 NZ_CP019905:c20809-20591 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGTACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References