Detailed information of TA system TA073469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 72037..72562
• Replicon: plasmid unnamed1
• Accession: NZ_CP019904
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: B1200_RS26445
• Protein ID: WP_004197633.1
• Coordinates: 72257..72562 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: B1200_RS26440
• Protein ID: WP_004197642.1
• Coordinates: 72037..72255 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS26390	67382..67756	+	375	WP_001348195.1	cupin domain-containing protein	-
B1200_RS26395	67780..68343	+	564	WP_001097412.1	chlorite dismutase family protein	-
B1200_RS26400	68594..69691	-	1098	Protein_71	IS66 family transposase	-
B1200_RS26410	69745..70449	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
B1200_RS26415	70496..70732	-	237	WP_001348075.1	AAA family ATPase	-
B1200_RS26420	70806..71222	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	-
B1200_RS26425	71219..71449	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
B1200_RS26430	71406..71828	+	423	WP_162781986.1	hypothetical protein	-
B1200_RS26440	72037..72255	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
B1200_RS26445	72257..72562	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
B1200_RS26450	72731..73132	+	402	WP_072199347.1	hypothetical protein	-
B1200_RS26455	73159..73482	+	324	WP_004197641.1	hypothetical protein	-
B1200_RS26460	73479..74495	+	1017	WP_004197639.1	hypothetical protein	-
B1200_RS26465	74693..75487	+	795	WP_004197635.1	site-specific integrase	-
B1200_RS30170	75574..75771	-	198	WP_021312488.1	hypothetical protein	-
B1200_RS26470	75927..76106	-	180	WP_006788217.1	hypothetical protein	-
B1200_RS26475	76226..76852	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108340	108340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T73469 WP_004197633.1 NZ_CP019904:72257-72562 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T73469 NZ_CP019904:72257-72562 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT73469 WP_004197642.1 NZ_CP019904:72037-72255 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT73469 NZ_CP019904:72037-72255 [Escherichia coli]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5