Detailed information of TA system TA073468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 70806..71449
• Replicon: plasmid unnamed1
• Accession: NZ_CP019904
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: B1200_RS26420
• Protein ID: WP_016236302.1
• Coordinates: 70806..71222 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: B1200_RS26425
• Protein ID: WP_001261282.1
• Coordinates: 71219..71449 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS26375	66176..66511	-	336	WP_000046891.1	thermonuclease family protein	-
B1200_RS26385	66754..67317	+	564	WP_001515734.1	transposase	-
B1200_RS26390	67382..67756	+	375	WP_001348195.1	cupin domain-containing protein	-
B1200_RS26395	67780..68343	+	564	WP_001097412.1	chlorite dismutase family protein	-
B1200_RS26400	68594..69691	-	1098	Protein_71	IS66 family transposase	-
B1200_RS26410	69745..70449	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
B1200_RS26415	70496..70732	-	237	WP_001348075.1	AAA family ATPase	-
B1200_RS26420	70806..71222	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
B1200_RS26425	71219..71449	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B1200_RS26430	71406..71828	+	423	WP_162781986.1	hypothetical protein	-
B1200_RS26440	72037..72255	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	-
B1200_RS26445	72257..72562	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
B1200_RS26450	72731..73132	+	402	WP_072199347.1	hypothetical protein	-
B1200_RS26455	73159..73482	+	324	WP_004197641.1	hypothetical protein	-
B1200_RS26460	73479..74495	+	1017	WP_004197639.1	hypothetical protein	-
B1200_RS26465	74693..75487	+	795	WP_004197635.1	site-specific integrase	-
B1200_RS30170	75574..75771	-	198	WP_021312488.1	hypothetical protein	-
B1200_RS26470	75927..76106	-	180	WP_006788217.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108340	108340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T73468 WP_016236302.1 NZ_CP019904:c71222-70806 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T73468 NZ_CP019904:c71222-70806 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT73468 WP_001261282.1 NZ_CP019904:c71449-71219 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT73468 NZ_CP019904:c71449-71219 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3