Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4595746..4596371 Replicon chromosome
Accession NZ_CP019903
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag B1200_RS24210 Protein ID WP_000911329.1
Coordinates 4595746..4596144 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag B1200_RS24215 Protein ID WP_000450524.1
Coordinates 4596144..4596371 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS24190 4591653..4591853 + 201 WP_000383836.1 YpfN family protein -
B1200_RS24195 4591934..4592632 - 699 WP_000679819.1 esterase -
B1200_RS24200 4592706..4594721 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
B1200_RS24205 4594736..4595599 - 864 WP_001267498.1 neutral zinc metallopeptidase -
B1200_RS24210 4595746..4596144 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
B1200_RS24215 4596144..4596371 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
B1200_RS24220 4596527..4597241 - 715 Protein_4415 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
B1200_RS24225 4597454..4598488 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
B1200_RS24230 4598505..4599383 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
B1200_RS24235 4599529..4600101 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
B1200_RS24240 4600101..4600571 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T73467 WP_000911329.1 NZ_CP019903:c4596144-4595746 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T73467 NZ_CP019903:c4596144-4595746 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT73467 WP_000450524.1 NZ_CP019903:c4596371-4596144 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT73467 NZ_CP019903:c4596371-4596144 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References