Detailed information of TA system TA073454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2839065..2839770
• Replicon: chromosome
• Accession: NZ_CP019903
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: B1200_RS14815
• Protein ID: WP_000539521.1
• Coordinates: 2839384..2839770 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B1200_RS14810
• Protein ID: WP_001280945.1
• Coordinates: 2839065..2839394 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS14795	2834222..2835133	+	912	WP_078081000.1	glutathione ABC transporter permease GsiD	-
B1200_RS14800	2835311..2837659	+	2349	Protein_2677	EAL domain-containing protein	-
B1200_RS14805	2837667..2838995	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
B1200_RS14810	2839065..2839394	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
B1200_RS14815	2839384..2839770	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B1200_RS14820	2839996..2841321	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
B1200_RS14825	2841534..2841917	+	384	WP_000497137.1	biofilm formation regulator BssR	-
B1200_RS14830	2842028..2843143	+	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
B1200_RS14835	2843140..2843766	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T73454 WP_000539521.1 NZ_CP019903:c2839770-2839384 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T73454 NZ_CP019903:c2839770-2839384 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT73454 WP_001280945.1 NZ_CP019903:c2839394-2839065 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT73454 NZ_CP019903:c2839394-2839065 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6