Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2424232..2424850 Replicon chromosome
Accession NZ_CP019903
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag B1200_RS12565 Protein ID WP_001291435.1
Coordinates 2424232..2424450 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag B1200_RS12570 Protein ID WP_000344800.1
Coordinates 2424476..2424850 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS12530 2419521..2420093 + 573 WP_000779821.1 YbaY family lipoprotein -
B1200_RS12535 2420124..2420435 - 312 WP_000409911.1 MGMT family protein -
B1200_RS12545 2420814..2421167 + 354 WP_000878140.1 DUF1428 domain-containing protein -
B1200_RS12550 2421209..2422759 - 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
B1200_RS12555 2422923..2423393 - 471 WP_000136192.1 YlaC family protein -
B1200_RS12560 2423509..2424060 - 552 WP_000102564.1 maltose O-acetyltransferase -
B1200_RS12565 2424232..2424450 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
B1200_RS12570 2424476..2424850 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
B1200_RS12575 2425396..2428545 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
B1200_RS12580 2428568..2429761 - 1194 WP_078080977.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73452 WP_001291435.1 NZ_CP019903:c2424450-2424232 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T73452 NZ_CP019903:c2424450-2424232 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT73452 WP_000344800.1 NZ_CP019903:c2424850-2424476 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT73452 NZ_CP019903:c2424850-2424476 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References