Detailed information of TA system TA073450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1977226..1977650
• Replicon: chromosome
• Accession: NZ_CP019903
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: B1200_RS30265
• Protein ID: WP_001306201.1
• Coordinates: 1977462..1977650 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0J2BBI0
• Locus tag: B1200_RS10305
• Protein ID: WP_000125563.1
• Coordinates: 1977226..1977459 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS10280	1972299..1972586	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
B1200_RS10285	1972645..1973976	+	1332	WP_001183198.1	MFS transporter	-
B1200_RS10290	1974084..1974614	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
B1200_RS10295	1974607..1976469	+	1863	WP_000377115.1	glutathione-regulated potassium-efflux system protein KefC	-
B1200_RS10300	1976661..1977140	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
B1200_RS10305	1977226..1977459	+	234	WP_000125563.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
B1200_RS30265	1977462..1977650	+	189	WP_001306201.1	CcdB family protein	Toxin
B1200_RS30270	1977647..1977763	+	117	WP_000148634.1	CcdB family protein	-
B1200_RS10315	1977760..1978608	-	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
B1200_RS10320	1978615..1978992	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
B1200_RS10325	1978995..1979816	-	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
B1200_RS10330	1979813..1980802	-	990	WP_000241249.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
B1200_RS10335	1980802..1982088	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7167.44 Da
• Isoelectric Point: 11.6113

>T73450 WP_001306201.1 NZ_CP019903:1977462-1977650 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T73450 NZ_CP019903:1977462-1977650 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8542.58 Da
• Isoelectric Point: 5.8062

>AT73450 WP_000125563.1 NZ_CP019903:1977226-1977459 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAAIRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT73450 NZ_CP019903:1977226-1977459 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAATACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0J2BBI0