Detailed information of TA system TA073449

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1818994..1819792
• Replicon: chromosome
• Accession: NZ_CP019903
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X1M0U2
• Locus tag: B1200_RS09480
• Protein ID: WP_000854919.1
• Coordinates: 1819415..1819792 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2G4AEZ5
• Locus tag: B1200_RS09475
• Protein ID: WP_001285608.1
• Coordinates: 1818994..1819368 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS09440	1814717..1815397	+	681	WP_001282921.1	WYL domain-containing protein	-
B1200_RS09445	1815545..1816222	+	678	WP_001097301.1	hypothetical protein	-
B1200_RS09450	1816228..1816461	+	234	WP_001278293.1	DUF905 family protein	-
B1200_RS09455	1816551..1817369	+	819	WP_078080903.1	DUF945 domain-containing protein	-
B1200_RS09460	1817635..1818114	+	480	WP_001696591.1	antirestriction protein	-
B1200_RS09465	1818130..1818606	+	477	WP_078080954.1	RadC family protein	-
B1200_RS09470	1818693..1818914	+	222	WP_000692350.1	DUF987 domain-containing protein	-
B1200_RS09475	1818994..1819368	+	375	WP_001285608.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
B1200_RS09480	1819415..1819792	+	378	WP_000854919.1	TA system toxin CbtA family protein	Toxin
B1200_RS09485	1819789..1820277	+	489	WP_000777665.1	hypothetical protein	-
B1200_RS09490	1820297..1820494	+	198	WP_001403937.1	DUF957 domain-containing protein	-
B1200_RS30260	1820579..1820725	+	147	WP_001290177.1	hypothetical protein	-
B1200_RS09500	1821678..1823715	+	2038	Protein_1710	hypothetical protein	-
B1200_RS09505	1824114..1824293	-	180	WP_001153034.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14037.97 Da
• Isoelectric Point: 7.9086

>T73449 WP_000854919.1 NZ_CP019903:1819415-1819792 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T73449 NZ_CP019903:1819415-1819792 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13610.37 Da
• Isoelectric Point: 5.5051

>AT73449 WP_001285608.1 NZ_CP019903:1818994-1819368 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPAITS

Nucleotide

• Length: 375 bp

>AT73449 NZ_CP019903:1818994-1819368 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAATCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A1X1M0U2

Antitoxin

• AlphaFold DB: A0A2G4AEZ5