Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1558599..1559096 Replicon chromosome
Accession NZ_CP019903
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag B1200_RS08080 Protein ID WP_001173343.1
Coordinates 1558923..1559096 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2X1N3L1
Locus tag B1200_RS08075 Protein ID WP_001560287.1
Coordinates 1558599..1558895 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS08055 1555329..1556048 - 720 WP_000611288.1 two-component system response regulator DcuR -
B1200_RS08060 1556045..1557676 - 1632 WP_001216477.1 sensor histidine kinase -
B1200_RS08065 1557856..1558086 + 231 WP_000371717.1 (4Fe-4S)-binding protein -
B1200_RS08070 1558098..1558370 + 273 WP_000405647.1 N-acetyltransferase -
B1200_RS08075 1558599..1558895 + 297 WP_001560287.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
B1200_RS08080 1558923..1559096 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
B1200_RS08085 1559215..1560732 - 1518 WP_000003806.1 lysine--tRNA ligase -
B1200_RS08090 1560969..1562426 - 1458 WP_000856819.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T73447 WP_001173343.1 NZ_CP019903:1558923-1559096 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T73447 NZ_CP019903:1558923-1559096 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11441.94 Da        Isoelectric Point: 4.2924

>AT73447 WP_001560287.1 NZ_CP019903:1558599-1558895 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT73447 NZ_CP019903:1558599-1558895 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGCATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1N3L1

References