Detailed information of TA system TA073445

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1017261..1018062
• Replicon: chromosome
• Accession: NZ_CP019903
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: B1200_RS05390
• Protein ID: WP_001094437.1
• Coordinates: 1017685..1018062 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XTQ1
• Locus tag: B1200_RS05385
• Protein ID: WP_001390338.1
• Coordinates: 1017261..1017638 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS05355	1013260..1013754	+	495	WP_078080932.1	antirestriction protein	-
B1200_RS05360	1013812..1014558	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
B1200_RS05365	1014573..1016114	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
B1200_RS05370	1016338..1016814	+	477	WP_001535682.1	RadC family protein	-
B1200_RS05375	1016877..1017098	+	222	WP_000692345.1	DUF987 domain-containing protein	-
B1200_RS05385	1017261..1017638	+	378	WP_001390338.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B1200_RS05390	1017685..1018062	+	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
B1200_RS05395	1018059..1018547	+	489	WP_000761716.1	hypothetical protein	-
B1200_RS05400	1018567..1018764	+	198	WP_000772032.1	DUF957 domain-containing protein	-
B1200_RS05405	1018849..1019694	+	846	WP_001274561.1	DUF4942 domain-containing protein	-
B1200_RS05415	1020287..1020784	+	498	WP_000509808.1	hypothetical protein	-
B1200_RS05420	1020951..1021874	+	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
B1200_RS05425	1021878..1022696	-	819	WP_000779405.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T73445 WP_001094437.1 NZ_CP019903:1017685-1018062 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T73445 NZ_CP019903:1017685-1018062 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13745.44 Da
• Isoelectric Point: 5.0463

>AT73445 WP_001390338.1 NZ_CP019903:1017261-1017638 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT73445 NZ_CP019903:1017261-1017638 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A0E0XTQ1