Detailed information of TA system TA073438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 736410..737041
• Replicon: chromosome
• Accession: NZ_CP019903
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: B1200_RS03920
• Protein ID: WP_001260301.1
• Coordinates: 736766..737041 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: B1200_RS03915
• Protein ID: WP_000593557.1
• Coordinates: 736410..736769 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS03890	732542..733486	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
B1200_RS03895	733483..734316	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
B1200_RS03900	734316..735080	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
B1200_RS03905	735077..735883	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
B1200_RS03910	735889..736290	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
B1200_RS03915	736410..736769	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B1200_RS03920	736766..737041	-	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
B1200_RS03925	737114..738238	-	1125	WP_001318088.1	ABC transporter permease	-
B1200_RS03930	738238..740973	-	2736	WP_032284871.1	ribosome-associated ATPase/putative transporter RbbA	-
B1200_RS03935	740970..742037	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T73438 WP_001260301.1 NZ_CP019903:c737041-736766 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T73438 NZ_CP019903:c737041-736766 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT73438 WP_000593557.1 NZ_CP019903:c736769-736410 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT73438 NZ_CP019903:c736769-736410 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1