Detailed information of TA system TA073432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4816198..4816814
• Replicon: chromosome
• Accession: NZ_CP019889
• Organism: Enterobacter hormaechei strain FRM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B1023_RS23755
• Protein ID: WP_017382676.1
• Coordinates: 4816198..4816569 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: B1023_RS23760
• Protein ID: WP_015569912.1
• Coordinates: 4816572..4816814 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1023_RS23740	4813698..4814600	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
B1023_RS23745	4814597..4815232	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
B1023_RS23750	4815229..4816158	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
B1023_RS23755	4816198..4816569	-	372	WP_017382676.1	type II toxin-antitoxin system VapC family toxin	Toxin
B1023_RS23760	4816572..4816814	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
B1023_RS23765	4817013..4817933	+	921	WP_017382675.1	alpha/beta hydrolase	-
B1023_RS23770	4817942..4818883	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
B1023_RS23775	4818928..4819365	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B1023_RS23780	4819362..4820243	-	882	WP_023298923.1	virulence factor BrkB family protein	-
B1023_RS23785	4820237..4820836	-	600	WP_045347175.1	glucose-1-phosphatase	-
B1023_RS23790	4820955..4821755	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T73432 WP_017382676.1 NZ_CP019889:c4816569-4816198 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T73432 NZ_CP019889:c4816569-4816198 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT73432 WP_015569912.1 NZ_CP019889:c4816814-4816572 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT73432 NZ_CP019889:c4816814-4816572 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3