Detailed information of TA system TA073431

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4044478..4045231
• Replicon: chromosome
• Accession: NZ_CP019889
• Organism: Enterobacter hormaechei strain FRM

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: B1023_RS19900
• Protein ID: WP_016154457.1
• Coordinates: 4044478..4044855 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B1023_RS19905
• Protein ID: WP_162838704.1
• Coordinates: 4044905..4045231 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1023_RS19865	4039611..4039925	-	315	WP_017382737.1	lipoprotein	-
B1023_RS19870	4040101..4040733	+	633	WP_017382736.1	response regulator transcription factor	-
B1023_RS19875	4040730..4042514	+	1785	WP_032671993.1	hybrid sensor histidine kinase/response regulator	-
B1023_RS19885	4042831..4043676	-	846	Protein_3788	DUF4942 domain-containing protein	-
B1023_RS19890	4043757..4043960	-	204	WP_009652505.1	DUF957 domain-containing protein	-
B1023_RS19895	4043990..4044481	-	492	WP_009652438.1	hypothetical protein	-
B1023_RS19900	4044478..4044855	-	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
B1023_RS19905	4044905..4045231	-	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B1023_RS19910	4045288..4045509	-	222	WP_009652392.1	DUF987 domain-containing protein	-
B1023_RS19915	4045523..4046005	-	483	WP_016154455.1	DNA repair protein RadC	-
B1023_RS19920	4046017..4046235	-	219	WP_009652503.1	hypothetical protein	-
B1023_RS19925	4046246..4046707	-	462	WP_045890390.1	antirestriction protein	-
B1023_RS19930	4046736..4046990	-	255	WP_009652519.1	hypothetical protein	-
B1023_RS19935	4046990..4047808	-	819	WP_009652424.1	DUF945 domain-containing protein	-
B1023_RS19940	4047912..4048145	-	234	WP_009652474.1	DUF905 domain-containing protein	-
B1023_RS19945	4048201..4048422	-	222	WP_009652443.1	hypothetical protein	-
B1023_RS19950	4048505..4048879	-	375	Protein_3801	hypothetical protein	-
B1023_RS19955	4048876..4049220	-	345	Protein_3802	HTH domain-containing protein	-
B1023_RS19960	4049482..4049778	-	297	WP_009652512.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T73431 WP_016154457.1 NZ_CP019889:c4044855-4044478 [Enterobacter hormaechei]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T73431 NZ_CP019889:c4044855-4044478 [Enterobacter hormaechei]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT73431 WP_162838704.1 NZ_CP019889:c4045231-4044905 [Enterobacter hormaechei]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT73431 NZ_CP019889:c4045231-4044905 [Enterobacter hormaechei]
ATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTTTGGCGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACGCGGATTTACGCCATCTCGACCAGGCATTCCCGC
TACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGTCTATCTCGCAATTTACCCGACACCTGCAACAAC
AGAATAA

Structures

Toxin

• AlphaFold DB: R8WN26