Detailed information of TA system TA073428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2624129..2624868
• Replicon: chromosome
• Accession: NZ_CP019889
• Organism: Enterobacter hormaechei strain FRM

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A3L9PBP9
• Locus tag: B1023_RS13065
• Protein ID: WP_003857133.1
• Coordinates: 2624383..2624868 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: B1023_RS13060
• Protein ID: WP_003857131.1
• Coordinates: 2624129..2624395 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1023_RS13035	2619710..2620432	+	723	WP_003857121.1	DUF969 domain-containing protein	-
B1023_RS13040	2620429..2621415	+	987	WP_017382341.1	DUF979 domain-containing protein	-
B1023_RS13045	2621425..2622420	+	996	WP_015570513.1	DUF2891 domain-containing protein	-
B1023_RS13050	2622442..2623830	-	1389	WP_045348254.1	MFS transporter	-
B1023_RS13055	2623904..2624065	+	162	Protein_2484	LysR family transcriptional regulator	-
B1023_RS13060	2624129..2624395	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
B1023_RS13065	2624383..2624868	+	486	WP_003857133.1	GNAT family N-acetyltransferase	Toxin
B1023_RS13070	2625041..2626444	+	1404	WP_000125668.1	ISNCY family transposase	-
B1023_RS13075	2626477..2627181	-	705	WP_000130816.1	arsenical resistance protein ArsH	-
B1023_RS13080	2627268..2627588	+	321	WP_000941305.1	transcriptional regulator	-
B1023_RS13085	2627634..2628923	+	1290	WP_000922628.1	arsenic transporter	-
B1023_RS13090	2628936..2629361	+	426	WP_000065802.1	glutaredoxin-dependent arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2625041..2626444	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17540.23 Da
• Isoelectric Point: 9.9658

>T73428 WP_003857133.1 NZ_CP019889:2624383-2624868 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T73428 NZ_CP019889:2624383-2624868 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCTTCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCC
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT73428 WP_003857131.1 NZ_CP019889:2624129-2624395 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT73428 NZ_CP019889:2624129-2624395 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A3L9PBP9

Antitoxin

• AlphaFold DB: A0A837FCR9