Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1647863..1648660 Replicon chromosome
Accession NZ_CP019889
Organism Enterobacter hormaechei strain FRM

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag B1023_RS07960 Protein ID WP_032647926.1
Coordinates 1648139..1648660 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag B1023_RS07955 Protein ID WP_015570876.1
Coordinates 1647863..1648132 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1023_RS07920 1643401..1643760 + 360 WP_017384779.1 helix-turn-helix transcriptional regulator -
B1023_RS07925 1643770..1643934 - 165 WP_017693668.1 helix-turn-helix transcriptional regulator -
B1023_RS07930 1644089..1645330 + 1242 WP_045333952.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
B1023_RS07935 1645363..1645590 - 228 WP_006809324.1 YccJ family protein -
B1023_RS07940 1645609..1646205 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
B1023_RS07945 1646597..1646767 + 171 WP_001273664.1 general stress protein -
B1023_RS07950 1646884..1647789 + 906 WP_017693666.1 DMT family transporter -
B1023_RS07955 1647863..1648132 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
B1023_RS07960 1648139..1648660 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
B1023_RS07965 1648719..1650041 - 1323 WP_045347301.1 pyrimidine utilization transport protein G -
B1023_RS07970 1650063..1650556 - 494 Protein_1502 pyrimidine utilization flavin reductase protein F -
B1023_RS07975 1650569..1651159 - 591 WP_032104810.1 malonic semialdehyde reductase -
B1023_RS07980 1651169..1651867 - 699 WP_118379316.1 alpha/beta fold hydrolase -
B1023_RS07985 1651875..1652261 - 387 WP_015570870.1 pyrimidine utilization protein C -
B1023_RS07990 1652273..1652962 - 690 WP_032653188.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T73420 WP_032647926.1 NZ_CP019889:1648139-1648660 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T73420 NZ_CP019889:1648139-1648660 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT73420 WP_015570876.1 NZ_CP019889:1647863-1648132 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT73420 NZ_CP019889:1647863-1648132 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References